New Chemical Probe Maps Stress-Responsive Protein Networks Beyond DNA Repair

A research team has developed a photo-clickable, chain-terminating nicotinamide adenine dinucleotide analogue, PCCT-NAD+, that enables systematic mapping of proteins interacting with poly(ADP-ribose), or PAR. When applied to cells exposed to oxidative stress, the platform identified 1,576 proteins with altered enrichment in the PAR-associated interactome, revealing particularly strong involvement of translation, ribosome organization, and protein quality-control pathways. The findings broaden th